Nitrogen Metabolism in Soybean Tissue Culture

Nitrogen Metabolism in Soybean Tissue Culture: II. Urea Utilization and Urease Synthesis Require Ni.

Potassium citrate (10 mM, pH 6) inhibits the growth of cultured (Glycine max L.) cells when urea is the sole nitrogen source. Ureadependent citrate toxicity is overcome by three separate additions to the growth medium: (a) NH(4)Cl (20 mM); (b) high levels of MgCl(2) (10 mM) or CaCl(2) (5-10 mM); (c) low levels of NiSO(4) (10(-2) mM). Additions of 10(-2) mM NiSO(4) not only overcome citrate grow...

Comparisons of soybean urease isolated from seed and tissue culture.

Urease was purified 500-fold to electrophoretic homogeneity from ground, dry soybeans. Sodium dodecyl sulfate-acrylamide gel electrophoresis indicates a subunit size of 93,500 daltons which is identical with that of jack bean urease. In solutions of high ionic strength, there exists a single urease species (species 1) with a size of about 480,000 daltons based on agarose column chromatography a...

Nitrogen metabolism in gastrointestinal tissue of the pig.

The protein content of the gastrointestinal tract is not more than 7 % of whole-body protein, while the amount of protein synthesized daily exceeds 20% of whole-body protein synthesis. Considering this active protein metabolism in the gastrointestinal tract as well as the role of the gastrointestinal tract in digestion and absorption, we have investigated fluxes of N and amino acids (AA) in rel...

Effect of rhizobium and nitrogen on soybean

Utilizing Soybean Milk to Culture Soybean Pathogens

Liquid and semi-solid culture media are used to maintain and proliferate bacteria, fungi, and Oomycetes for research in microbiology and plant pathology. In this study, a comparison was made between soybean milk medium, also referred to as soymilk, and media traditionally used for culturing soybean pathogens to determine if soymilk medium was an effective medium for growth of Colletotrichum tru...